How are the clotting assays performed on your coagulation factors?

21 Nov 2009

Support Center » Knowledgebase » How are the clotting assays performed on your coagulation factors?

	How are the clotting assays performed on your coagulation factors?

Solution Standard Curve:
An eight point standard curve is generated using the MLA 1400C. NPP Plasma (made and characterized in house) dilution is started at a 1:5 and subsequently serial diluted. To this dilution, an equal volume of FVII deficient plasma is added (1:1). The dilution is incubated at 37ºC for 106 seconds. Clotting is initiated by addition of 2 parts PT reagent [Hemoliance RecombiPlasTin; Lexington, MA]. A new standard curve was generated for each testing day.

Test Samples:
The purified samples are prepared by making a dilutions into FVII or FIX deficient plasma. These samples are then serially diluted in Assay Buffer by the MLA 1400C from a 5-fold dilution for a total of three dilutions. The MLA 1400C compares the clot times to the standard curve and expresses the result as a percentage on the printout.

Article Details

Article ID:

Created On:

29 Sep 2008 09:54 AM